Biosynthesis of the polymeric gel-forming mucin MUC5B
نویسندگان
چکیده
منابع مشابه
Biosynthesis of the polymeric gel-forming mucin MUC5B
MUC5B is a major polymeric mucin in the airway mucus gel and is an essential component of innate defense of the respiratory epithelium. Knowledge of the synthesis and intracellular processing of MUC5B is incomplete. We investigated the molecular details of MUC5B assembly in primary human bronchial epithelial cells (HBECs) grown at an air-liquid interface (ALI). Electrophoretic and centrifugal s...
متن کاملIn vivo imaging of the Muc5b gel-forming mucin
Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b production in mouse tissues by immunofluorescence microscopy and fluorescent activity using stereromicro...
متن کاملConcentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva.
We have developed a new approach to study the molecular organization of salivary mucus and salivary mucins using confocal fluorescence recovery after photobleaching (confocal-FRAP). MUC5B mucin, its reduced subunit and T-domains were prepared from saliva and fluorescently labelled. The translational self-diffusion coefficients were determined up to 3.6 mg/ml by confocal-FRAP. The results sugges...
متن کاملUnpacking a gel-forming mucin: a view of MUC5B organization after granular release.
Gel-forming mucins are the largest complex glycoprotein macromolecules in the body. They form the matrix of gels protecting all the surface epithelia and are secreted as disulfide-bonded polymeric structures. The mechanisms by which they are formed and organized within cells and thereafter released to form mucus gels are not understood. In particular, the initial rate of expansion of the mucins...
متن کاملMUC5B is the major mucin in the gel phase of sputum in chronic obstructive pulmonary disease.
RATIONALE Overproduction of mucus is a contributory factor in the progression of chronic obstructive pulmonary disease (COPD). The polymeric mucins are major macromolecules in the secretion. Therefore, we hypothesized that the polymeric mucin composition or properties may be different in the sputum from individuals with COPD and smokers without airflow obstruction. OBJECTIVES To determine the...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Lung Cellular and Molecular Physiology
سال: 2016
ISSN: 1040-0605,1522-1504
DOI: 10.1152/ajplung.00046.2016